Optimizing Pseudomonas Aeruginosa Treatment Case Study

Pseudomonas aeruginosa is a significant contributor to hospital acquired infections where delay of targeted treatment, including antimicrobial therapies, is associated with increased morbidity and mortality.1 Unlike porphyrin-producing bacteria which are visualized by a red fluorescence signal on the MolecuLight i:X, Pseudomonas aeruginosa uniquely produces a cyan color fluorescence signal due to intrinsic pyoverdine pigments.

Clinician's Testimonial

"I suspected colonization, and MolecuLight i:X guided me to efficiently target debridement and topical therapy application to ensure maximum removal of the bacterial load and prevent further contamination."

Rose Raizman, RN-EC, MSc, Scarborough & Rouge Hospital, Toronto, ON, Canada

Clinician Profile

Rose Raizman, RN-EC, MSc, with over 19 years of experience, leads the Save Our Skin (SOS) team at Scarborough & Rouge Hospital located in Toronto, Canada, to combat pressure ulcers of hospital inpatients. She also oversees the wound care clinic for inpatients and outpatients.

Clinical Synopsis

Patient Condition: 78 year old female patient with a venous leg ulcer received local wound care and negative pressure wound therapy. Against the clinician’s orders, home care nurses stopped using a prescribed antimicrobial paste. After guided fluorescence visualization of the wound, the clinician added larger silver dressings and antimicrobial cream to the patient’s treatment.

The clinician used the MolecuLight i:X to confirm the presence and location of Pseudomonas aeruginosa in a venous leg ulcer. As seen in Figure 3, the white/cyan color suggests heavy levels of Pseudomonas aeruginosa pre-debridement (the white/cyan color is due to signal oversaturation), which would otherwise be invisible to the unaided eye. The clinician used this information to guide debridement of the wound to remove bacteria-colonized tissues, and subsequently re-imaged the wound after debridement to assess effectiveness of the procedure.

Figure 1: ST-image
Venous leg ulcer pre-debridement 
Figure 2: FL-image
Venous leg ulcer pre-debridement; white/cyan regions suggest heavy levels of Pseudomonas aeruginosa 

Figure 4 indicates a reduction in overall wound bioburden post-debridement, but not the complete eradication of Pseudomonas aeruginosa in the wound. The MolecuLight i:X images (Figure 4) provided important evidence that Pseudomonas aeruginosa was still present after debridement, which helped the clinician make the decision at the point of care to select antimicrobial (silver) cream and dressings as well as applying a larger dressing to fully manage the remaining bacterial load.

Figure 3: ST-image
Figure 4: FL-image
Post-debridement, less white observed suggesting a reduction in bioburden but cyan fluorescence remains suggesting continued presence of Pseudomonas aeruginosa

This case shows how the MolecuLight i:X may provide important insights about the location of bacteria so clinicians can target debridement and treat wounds more effectively, compared to the standard of care.

At a Glance

Wound etiology & location Venous leg ulcer, left leg
Patient demographics Female, 78 years old
Patient-related challenges Home care nurses had stopped using prescribed antimicrobial against clinician orders
Patient’s general care paradigm

Antimicrobial paste


Local wound care

Clinician stated utility of the MolecuLight i:X

Visualization of periwound bacteria led to larger cut dressings

Guided selection of antimicrobial (silver) cream and dressings


Images provided by Rose Raizman, RN-EC, MSc, Scarborough & Rouge Hospital, Toronto, ON, Canada.

MolecuLight Clinical Case 0048.

  1. Sader HS et al. Pseudomonas aeruginosa Antimicrobial Susceptibility Results from Four Years (2012 to 2015) of the International Network for Optimal Resistance Monitoring Program in the United States. Antimicrob. Agents Chemother. March 2017; 61(3):e02252-16.

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