How does the MolecuLight i:X work?

The MolecuLight i:X emits a precise wavelength of safe violet light, which interacts with the wound tissue and bacteria causing the wound and surrounding skin to emit a green fluorescence (i.e. collagen) and potentially harmful bacteria to emit a red fluorescence (i.e. porphyrins).1

Which types of bacteria can be detected?

Evidence from clinical studies indicate that the MolecuLight i:X can detect2 the following bacterial species that are relevant for clinical wound care:

  • Staphylococcus aureus
  • Methicillin-Resistant Staphylococcus aureus (MRSA)
  • Enterobacter cloacae
  • Enterococcus faecalis
  • Proteus mirabilis
  • Klebsiella pneumoniae
  • Escherichia coli
  • Beta-hemolytic Streptococci (Group B)
  • Coagulase-negative Staphylococci (e.g. S. lugdunensis)
  • Pseudomonas aeruginosa

What are the clinical benefits of using the MolecuLight i:X?

The MolecuLight i:X can be used at the point of care to help you optimize all wound protocols including improved accuracy of sampling, more effective wound cleaning and debridement, as well as helping with dressing and antimicrobial selection.1,3,4,5,6 Not only can it help you optimize these protocols, you can also track how well your treatment plan is working to decrease bacterial load and wound size so you can modify the treatment plan if the patient is not responding to the current treatment plan.7,8

How easy is the MolecuLight i:X to use?

The MolecuLight i:X is as simple to use as your iPhone or any smart phone device.
The user interface on the device was designed to be intuitive and easy to use, without requiring a technical background.

Why does the device need to be used in the dark?

Room lighting can interfere with the fluorescence signal emitted from the bacteria that you’re trying to see. An ambient light sensor was implemented to inform the user if the room is dark enough.

What does it mean that the device shows ‘real-time’ data? How long does it take to get an image?

The bacteria fluorescence shows up immediately on the screen when FL-mode is activated in a dark environment, similar to a point a shoot and camera where you can see what the image will look like on the screen before capturing the image. No data processing or contrast agents are required. Fluorescence is instantaneous as long as Fluorescence Imaging Mode is on and room light is dark.

Is the device safe with laser light exposure and violet light illumination?

Yes, the device is classified in Risk Group 1 and the laser is Class 1 meaning there is not sufficient energy produced to damage skin or eyes in normal use. It is advised to not point the device towards the eyes.

Can I miss the fluorescence signal if there is blood in the wound?

Blood does not fluoresce. It also preferentially absorbs the excitation light thereby reducing the likelihood of (bacteria/tissue) fluorescence being generated. Bacteria covered by surface blood may be missed during FL-imaging, but this is rare as most users remove blood during wound assessment (when possible). It is recommended that FL-imaging be performed after (surface) blood has been removed from the wound bed and periwound areas.

How deep can the device see bacteria beneath the skin?

The MolecuLight i:X can detect bacteria at and below the wound surface (typically to 1.5 mm deep). When a wound is debrided using FL-image guidance, the clinician may discover bacteria in deeper parts of the wound.

How do I transfer images?

You can transfer the images from your device to any computer using the white MolecuLight i:X Connecting Cable. When plugged in, follow any prompts on the MolecuLight i:X Display Screen. The MolecuLight i:X will show up similar to a standard USB drive, where images/videos can easily be copied to the hard drive.


 If you have additional questions, please contact us.



  1. DaCosta RS, Kulbatski I, Lindvere-Teene L, Starr D, Blackmore K, Silver JI, Opoku J, Wu YC, Medeiros PJ, Xu W, Xu L, Wilson BC, Rosen C, Linden R. Point-of-care autofluorescence imaging for real-time sampling and treatment guidance of bioburden in chronic wounds: first-in-human results. PLOS ONE, 2015, 10(2). doi: 10.1371/journal.pone.0116623.
  2. Rennie MY, Lindvere-Teene L, Tapang K, Linden R. Point-of-care fluorescence imaging predicts the presence of pathogenic bacteria in wounds: a clinical study. Journal of Wound Care, 2017, 26(8), 452-460. doi: 10.12968/jowc.2017.26.8.452.
  3. Wu YC, Smith M, Chu A, Lindvere-Teene L, Starr D, Tapang K, Wong O, Linden R, DaCosta RS. Handheld fluorescence imaging device detects subclinical wound infection in an asymptomatic patient with chronic diabetic foot ulcer: a case report. International Wound Journal, 2016, 13(4), 449-53. doi: 10.1111/iwj.12451.
  4. Chamma E, Qiu J, Lindvere-Teene L, Blackmore KM, Majeed S, Weersink R, Dickie CI, Griffin AM, Wunder JS, Ferguson PC, DaCosta RS. Optically-tracked handheld fluorescence imaging platform for monitoring skin response in the management of soft tissue sarcoma. Journal of Biomedical Optics, 2015, 20(7). doi: 10.1117/1.JBO.20.7.076011.
  5. Hoeflok J, Teene L, Chamma E, Chu A, DaCosta RS. Pilot clinical evaluation of surgical site infections with a novel handheld fluorescence imaging device. Presented at MHSRS 2014. Proceedings of the Annual Military Health System Research Symposium; 2014 Aug 18-21; Fort Lauderdale, FL, USA.
  6. Landis S, Rennie MY, Blumenthal E, Jeffery S. Use of fluorescence imaging in visualizing bacteria in chronic ulcers and traumatic soft tissue damage. Presented at AMSUS 2016. Proceedings of the Annual Meeting of the Society of Federal Health Professionals; 2016 Nov 29-Dec 2; National Harbor, MD, USA.
  7. Raizman R. Point-of-care fluorescence imaging device guides care and patient education in obese patients with surgical site infections. Presented at CAWC 2016. Proceedings of the Annual Canadian Association of Wound Care Conference; 2016 Nov 3-6, Niagara Falls, ON, Canada.
  8. Landis S. Mapping venous ulcers using bacterial autofluorescence (BAF) to identify subgroups at risk of infection post debridement. Presented at CAWC 2016. Proceedings of the Annual Canadian Association of Wound Care Conference; 2016 Nov 3-6, Niagara Falls, ON, Canada.