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Evidence Category:
Wound Sampling

Wound Sampling

Fluorescence images provided the clinician with a “map” to inform wound sampling, cleaning, and location of surgical debridement

Heavy bacterial load was confirmed by microbiology methods in all wounds exhibiting red fluorescence

Fluorescence images identified asymptomatic patients and highlighted areas of clinical concern that otherwise would have been overlooked


The PPV of red fluorescence on MolecuLight i:X images was 100%, regardless of sampling method, analysis technique, or study site

Fluorescence guidance, in combination with subsurface sampling techniques, could eliminate the risk of false negative wound sampling

Fluorescence imaging information could influence treatment decisions at the point of care


Fluorescence imaging of diabetic foot ulcers had superior accuracy (78%) in detecting the presence of clinically significant bacteria than standard practice (Levine swabbing, 52%; p=0.048)

Higher bacterial loads were detected from wound regions positive for bacterial fluorescence compared to regions sampled based on CSS alone