Back to All Clinical Evidence
Evidence Category:
Chronic Wounds

pH values on wound bed confirm non-invasive correlation between fluorescence & bacterial burden

MolecuLight i:X is able to objectively detect the bacterial proliferation in chronic wounds

MolecuLight also guides the correct assessment in the absence of clinical signs of infection and the presence of subclinical bacteria burden


Reliance on CSS to diagnose clinically significant bacterial burden in chronic wounds leads to the haphazard use of antimicrobials

Improved methods of identifying bacterial burden and infection are needed to enhance antimicrobial stewardship efforts in wound care

MolecuLight i:X can provide utility in point-of-care detection of elevated bacterial burden  


This study aimed to assess the efficacy and safety of a porcine peritoneum-derived matrix, a type of cellular and/or tissue product for treatment of DFUs

MolecuLight i:X imaging and measurement of bacterial protease activity (BPA) were performed weekly to evaluate bacterial load in wounds

Low bacterial loads, indicated by absence of fluorescence on MolecuLight i:X images and negative BPA, predicted wound healing


Fluorescence imaging was used to meet Joint Commission guidelines for an antimicrobial stewardship program

Combined with clinical examination of signs and symptoms of infection, the MolecuLight imaging procedure improves the clinician’s ability to diagnose infection

Antimicrobial stewardship will soon become part of standard wound center reporting


This RCT used fluorescence imaging to evaluate the effects of cold atmospheric plasma on wound area of chronic wounds

Fluorescence images were used to visualize the bacterially colonized area and compare the area before and after

50.4 % reduction of bacterial load for Group 1 and 35.0 % for Group 2 compared to the day of study


24% of VLU periwound area was colonized by high bacterial loads pre-debridement

99% reduction of bacterial signal in the wound bed after debridement using fluorescence guidance

36% of periwound bacterial signal is left behind after standard debridement in VLUs (without
the guidance of fluorescence)