Abstract: New concepts and strategies for managing wound infection, biofilm and impaired wound healing are urgently needed. This study examines the effects of non-thermal gas plasma (NTGP) in the management of diabetic foot ulcers stalled by subclinical, biofilm-related wound infection. Patients either received local standard of care or standard care plus 5 minutes NTGP twice weekly. This included using the MolecuLight i:X to assess the presence and location of elevated bacteria (>104 CFU/g) in the wounds. Qualitative analysis of assessed parameters (wound closure, bioburden, inflammation) showed that overall outcomes were good to very good. The data obtained strengthens the evidence of the beneficial effect of NTGP on wound healing in patients with diabetic foot ulcers.