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Evidence Category:
Species Detected/Biofilm

Pseudomonas aeruginosa, a common bacterial pathogen in chronic wounds, is challenging to detect by standard assessment of clinical signs and symptoms

Cyan detected on MolecuLight i:X fluorescence images, can be used to reliably predict Pseudomonas aeruginosa at the point-of-care, with a PPV of 93% (confirmed by microbiological analysis)


In this prospective trial, addition of a biofilm disrupting antimicrobial agent to NPWT reduced surface area and led to improved wound healing in 4/6 recalcitrant pressure ulcers

A reduction in wound area correlated with a reduction in bacterial burden as evidenced by fluorescence imaging (MolecuLight i:X)

The MolecuLight procedure enabled detection of bacteria build up in the NPWT sponges at the point-of-care


Successful removal of biofilm in wounds is contingent upon accurate detection of bacterial burden

Fluorescence imaging (MolecuLight i:X) can detect wound bacteria encased in biofilm, as confirmed by SEM and histopathology

Red fluorescence observed from in vivo polymicrobial wound models is specific to bacteria, and not from an immune response


Fluorescence imaging was used to inform on the presence of bacterial loads in stage 3 and 4 pressure ulcers

These pressure ulcers underwent NPWT and treatment with a biofilm disrupting agent

In wounds where negative fluorescence images suggested lower bacterial levels, a reduction in wound surface area and volume was observed