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Species Detected/Biofilm, Pre-Clinical Applications

In vitro detection of porphyrin-producing wound bacteria with real-time fluorescence imaging

Fluorescence imaging (MolecuLight i:X) can detect both planktonic & biofilm bacterial cultures

Biofilm is present in more than 70% of chronic wounds and it contributes to delayed healing

Using fluorescence imaging to localize bacteria may facilitate targeted disruption of biofilm & bacterial removal

Aim: Fluorescence imaging (MolecuLight i:X® real-time fluorescence imaging device) can visualize polymicrobial populations in chronic and acute wounds based on porphyrin fluorescence. We investigated the fluorescent properties of specific wound pathogens and the fluorescence detected from bacteria in biofilm.

Methods: Utilizing Remel Porphyrin Test Agar, 32 bacterial and four yeast species were examined for red fluorescence under 405 nm violet light illumination. Polymicrobial biofilms, supplemented with δ-aminolevulinic acid, were investigated similarly.

Results: A total of 28/32 bacteria, 1/4 yeast species and polymicrobial biofilms produced red fluorescence, in agreement with their known porphyrin production abilities.

Conclusion: These results identify common wound pathogens capable of producing porphyrin-specific fluorescence and support clinical observations using fluorescence imaging (with the MolecuLight i:X) to detect pathogenic bacteria in chronic wounds.

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1MolecuLight Inc., Toronto, ON, Canada
2Texas Tech University, Lubbock, USA
3University Health Network/Sinai Health System, Toronto, ON, Canada
4Princess Margaret Cancer Center, University Health Network, Toronto, Canada