Visualize Bacteria

See what you’re missing with MolecuLight i:

The MolecuLight i:X is a handheld fluorescence imaging device that provides instant visual detection and documentation of potentially harmful bacteria in wounds that would otherwise be invisible.1 The MolecuLight i:X emits a precise wavelength of safe violet light, which interacts with the wound tissue and bacteria causing the wound and surrounding skin to emit a green fluorescence (i.e. collagen) and potentially harmful bacteria to emit a red fluorescence (i.e. porphyrins).1 In real-time, MolecuLight i:X captures these red and green fluorescence signals using specialized optical components to filter out the violet light, and displays the resultant image immediately on the display screen (FL-image).1,2 The MolecuLight i:X is precisely calibrated to detect fluorescent bacteria at levels of ≥ 104 CFU/g on a quantitative scale or predominantly moderate to heavy growth on a semi-quantitative scale.3

Leveraging the principle of fluorescence, MolecuLight i:X emits a safe violet light which causes bacteria ≥ 104 CFU/g to fluoresce


Which types of bacteria can be detected?

Evidence* from clinical studies indicate that the MolecuLight i:X can detect3 the following bacterial species that are relevant for clinical wound care:

  • Staphylococcus aureus
  • Methicillin-Resistant Staphylococcus aureus (MRSA)
  • Enterobacter cloacae
  • Enterococcus faecalis
  • Proteus mirabilis
  • Klebsiella pneumoniae
  • Escherichia coli
  • Beta-hemolytic Streptococci (Group B)
  • Coagulase-negative Staphylococci (e.g. S. lugdunensis)
  • Pseudomonas aeruginosa



*Bacterial presence confirmed by microbiological analysis (qPCR: bacterial load ≥ 104 CFU/g; culture analysis: bacterial load predominately ≥ moderate/heavy)

  1. DaCosta RS, Kulbatski I, Lindvere-Teene L, Starr D, Blackmore K, Silver JI, Opoku J, Wu YC, Medeiros PJ, Xu W, Xu L, Wilson BC, Rosen C, Linden R. Point-of-care autofluorescence imaging for real-time sampling and treatment guidance of bioburden in chronic wounds: first-in-human results. PLOS ONE, 2015, 10(2). doi: 10.1371/journal.pone.0116623.
  2. Raizman R, DaCosta RS. Handheld real-time fluorescence imaging of bacteria guides treatment selection and timing of dressing changes in inpatients undergoing negative pressure wound therapy.  Presented at IWH 2016. Proceedings of the Innovations in Wound Healing Conference; 2016 Dec 8-11, Key Largo, FL, USA.
  3. Rennie MY, Lindvere-Teene L, Tapang K, Linden R. Point-of-care fluorescence imaging predicts the presence of pathogenic bacteria in wounds: a clinical study. Journal of Wound Care, 2017, 26(8), 452-460. doi: 10.12968/jowc.2017.26.8.452.